Excavator No 18

  • CHTF8 - Chromosome transmission fidelity protein 8 homolog

    Ctf18-RFC, which consists of the chromosome cohesion factors Ctf18, Dcc1, and Ctf8 and four small RFC subunits, functions as a second proliferating cell nuclear antigen (PCNA) loader. To identify potential targets of Ctf18-RFC, human cell extracts were assayed for DNA polymerase activity specifically stimulated by Ctf18-RFC in conjunction with PCNA.

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  • Human CTF18-RFC clamp-loader complexed with non

    Feb 13, 2017 · The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ε (Polε). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Polε.

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  • A second proliferating cell nuclear antigen loader complex

    May 31, 2007 · A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity. Yasushi Shiomi Department of Biology, School of Sciences, Kyushu University, Kefid Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

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  • Control of Genome Integrity by RFC Complexes; Conductors

    In eukaryotes, the DNA sliding clamp, proliferating cell nuclear antigen (PCNA), acts on chromatin as a processivity factor for DNA polymerases. Since its discovery, many other PCNA binding partners have been identified that function during DNA replication, repair, recombination, chromatin remodeling, cohesion, and proteolysis in cell-cycle

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  • Functions of Multiple Clamp and Clamp-Loader …

    Nov 05, 2010 · One of the proliferating cell nuclear antigen loader complexes, Ctf18-replication factor C (RFC), is involved in sister chromatid cohesion. To examine its relationship with factors involved in DNA replication, we performed a proteomics analysis of Ctf18-interacting proteins.

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  • CTF18 interacts with replication protein A in response to

    May 13, 2016 · Shiomi Y, Masutani C, Hanaoka F, Kimura H and Tsurimoto T: A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity. J Biol Chem. 282:20906–20914. 2007. View Article: Google Scholar: PubMed/NCBI. 12

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  • CTF18 interacts with replication protein A in response to

    May 13, 2016 · Shiomi Y, Masutani C, Hanaoka F, Kimura H and Tsurimoto T: A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity. J Biol Chem. 282:20906–20914. 2007. View Article: Google Scholar: PubMed/NCBI. 12

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  • ReplicationFactorCIsaMoreEffectiveProliferatingCell

    unit (Rfc1) of the complex is replaced by another protein sub-unit such as Rad24 in Saccharomyces cerevisiae (Rad17 in humans), Ctf18, or Elg1 to form their respective RFC com-plexes, Rad24-RFC, Ctf18-RFC, and Elg1-RFC. Rad24-RFC functions as part of a DNA damage checkpoint pathway. This clamp loader loads an alternative clamp, the Liming complex,

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  • CHTF18 - Wikipedia

    • DNA clamp loader activity: Cellular component • membrane • Ctf18 RFC-like complex • cell nucleus • nucleoplasm • cytosol: Biological process • DNA replication • cell cycle • positive regulation of DNA-directed DNA polymerase activity: Sources:Amigo / QuickGO

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  • CHTF8 chromosome transmission fidelity factor 8 [ …

    Shiomi Y, Masutani C, Hanaoka F, Kimura H, Tsurimoto T (Jul 2007). "A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity". The Journal of Biological Chemistry. 282 (29): 20906–14. doi: 10.1074/jbc.M610102200.

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  • Recombinant Human PCNA Protein (Functional) (A476

    Proliferating cell nuclear antigen (PCNA; a homotrimeric protein that encircles double-stranded DNA to function as a sliding clamp for DNA polymerases) is monoubiquitylated by the RAD6-RAD18 complex and further polyubiquitylated by the RAD5-MMS2-UBC13 complex …

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  • Stable Interaction between the Human Proliferating Cell

    Nov 05, 2010 · Introduction. The eukaryotic loader complex replication factor C (RFC) 2 consists of one large and four small subunits (RFC1 and RFC2–5, respectively) and loads the clamp complex proliferating cell nuclear antigen (PCNA) onto a primer/template DNA through its ATPase activity. PCNA is a homotrimeric complex functioning as the processivity factor for replicative DNA polymerase δ (pol …

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  • A second proliferating cell nuclear antigen loader complex

    Ctf18-RFC, which consists of the chromosome cohesion factors Ctf18, Dcc1, and Ctf8 and four small RFC subunits, functions as a second proliferating cell nuclear antigen (PCNA) loader. To identify potential targets of Ctf18-RFC, human cell extracts were assayed for DNA polymerase activity specifically stimulated by Ctf18-RFC in conjunction with

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  • Eukaryotic clamp loaders and unloaders in the maintenance

    Dec 18, 2020 · Murakami, T. et al. Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-replication factor C (RFC) and DNA polymerase {epsilon} is …

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  • The alternative Ctf18-Dcc1-Ctf8-replication factor C

    Dec 06, 2016 · This gene encodes a short protein that forms part of the Ctf18 replication factor C (RFC) complex that occurs in both yeast and mammals. The heteroheptameric RFC complex plays a role in sister chromatid cohesion and may load the replication clamp PCNA (proliferating cell nuclear antigen) onto DNA during DNA replication and repair. This gene is ubiquitously expressed and has been shown …

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  • The reconstituted human Chl12‐RFC complex functions as a

    Jun 15, 2010 · The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated the presence of primed DNA, replication protein A (RPA) and proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA.

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  • CHTF18 - Chromosome transmission fidelity protein 18

    Mar 01, 2002 · Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity …

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  • CHTF18 - Wikipedia

    Apr 02, 2004 · The sister chromatid cohesion factor Chl12 shares amino acid sequence similarity with RFC1, the largest subunit of replication factor C (RFC), and forms a …

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  • The reconstituted human Chl12-RFC complex functions as a

    Ctf18-RFC, which consists of the chromosome cohesion factors Ctf18, Dcc1, and Ctf8 and four small RFC subunits, functions as a second proliferating cell nuclear antigen (PCNA) loader.

    Learn More
  • Human CTF18-RFC clamp-loader complexed with non

    Feb 13, 2017 · The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ε (Polε). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Polε.

    Learn More